FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHE1244_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHE1244_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831935
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA38360.2093960757341281No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC33950.18532316921724842No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC26890.14678468395439795No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC26400.14410991656363353No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA21590.11785352646245636No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC21420.11692554593912995No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA20280.11070261772388211No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT20250.11053885645505983No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA18410.1004948319672914No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG18320.10000354816082449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7600.092.563081
GAGCAAA34600.080.488671
AGCGAAA12550.062.410791
AGCAAAA45000.061.0710222
CGAAAGC13250.060.1984443
GCGAAAG13250.059.1039282
CAAAAGC47400.058.7388954
GCAAAAG48950.055.994783
AAAGCAG68650.050.379146
TGTCCGA450.00964150548.33194145
AAAAGCA59950.047.409985
CGTCAGA951.9196195E-645.7969062
TATCGTG15650.044.0167272
CGTGTAG15500.043.9712755
TCGTGTA15850.043.00034
ATCGTGT15900.042.868593
CGCAGTC3400.042.6504865
CAGTCGG7150.041.572237
ATATCGT16600.041.0676081
GCAGTCG7450.039.9025346
Produced by FastQC (version 0.11.5)