FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHE1201_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHE1201_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545932
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT30170.11850277226571646No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA28500.11194328835177059No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT28230.11088277298843803No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG25970.10200586661387656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG47900.062.5186423
GAGCGAA11850.060.5966151
CGAAAGC15850.058.556583
TCACGCA2600.055.7765542
AGCGAAA17650.051.779441
GCGAAAG19050.049.1009332
AGCAAAA61850.048.8867722
GAGCAAA64800.047.795211
CAAAAGC67850.045.2049034
AAGCAGG86100.043.3632977
CGACCAC700.001057185941.426685145
GTCGGCT6300.040.2759489
TAGAAAC20950.039.456252
TATCGTG10450.038.1629032
AAAGCAG101100.036.8643846
GCAGTCG6900.036.780196
CGTGTAG11000.036.2547575
ACGCAGT4050.035.8071674
TTAGACA8950.034.0268144
GTAGAAA25400.033.9816741
Produced by FastQC (version 0.11.5)