FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHE1171_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHE1171_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981298
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA19850.20228309850830228No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC15470.15764833924047536No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC12380.1261594337296112No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC12300.12534418698499336No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC11260.11474597930496139No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA10090.10282299566492542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4400.0100.538421
GAGCAAA23100.074.717091
CGTGTAG6700.062.7652975
TATCGTG7000.062.1469232
AGCAAAA27200.061.8422782
CAAAAGC27700.060.9877434
TCGTGTA7050.060.6777234
GCAAAAG28150.059.497673
AGCGAAA7400.058.7876242
ATATCGT7300.058.6116681
ATCGTGT7350.058.2010843
CGAAAGC8000.054.3785554
AAAGCAG39550.053.3473556
GCGAAAG8350.052.0992133
CAGTCGG2900.049.998177
AAAAGCA34850.048.8912855
GCGATGT450.00964047448.3315669
AAGCAGG44250.047.840067
GCAGTCG2750.047.457656
GTGTAGA14300.046.6464046
Produced by FastQC (version 0.11.5)