FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHE1165_2_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHE1165_2_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130051
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC34930.16398668388691162No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA34140.16027785250212317No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC33420.15689765174636663No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC26810.12586553091921274No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA24310.11412872273950249No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA24040.11286114745609377No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC23890.11215693896531116No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC21380.10037318355288208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGC203.8669875E-4108.747718
GAGCGAA8050.095.5131451
GAGCAAA33050.083.399861
AGCGAAA14700.073.522821
GCGAAAG16050.067.316522
CGAAAGC17050.063.368343
AGCAAAA46350.059.4489632
CAAAAGC51000.053.8839044
CGCAGTC3600.052.367395
GCAAAAG54000.051.6984373
AAAGCAG70000.050.445336
CGTGTAG13800.048.8645485
CCGGTTG450.00963426948.3413963
TCGTGTA14100.047.824884
ATCGTGT14550.046.84633
AAAAGCA66000.042.2967345
TATCGTG18150.041.549632
TCACGCA4400.041.200052
TACGACG2300.040.9850963
TTACGAC2150.040.4718672
Produced by FastQC (version 0.11.5)