FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_BHE1039_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_BHE1039_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585268
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA77110.29826694950001315No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT76530.2960234683599534No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA52120.20160385693088687No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT50350.19475737138277346No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG44790.17325089700564894No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA44400.1717423493425053No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43560.16849316976034978No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG42230.1633486354219369No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC42090.16280710549157765No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA41480.16044758222358377No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT39080.15116421198885377No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC37170.14377619651038115No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT33550.12977377973966336No Hit
GATCCTCATGATCTCAGAGAACTCCTCTTTCTTAATCCTTCCAGACTCGA33420.12927093051861546No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC31470.12172819220289734No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC30420.11766671772520296No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG30130.11654497715517308No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC29610.11453358027098158No Hit
CCCTTATACTGGAGACCCTCCATACAGCCATGGAACAGGGACAGGGTACA29480.1140307310499337No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA29450.11391468892199957No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAATTACGGAATCTGATGTCACA29290.11329579757301757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG100.0070893285145.014914
GAGCGAA12400.099.438091
AGTAGAA12950.091.2942351
CAAAAGC72350.077.468234
GCAAAAG76800.071.756333
TTGTGTA1050.069.0547264
AAGCAGG105900.064.077237
GCAGGGG5400.060.41479
AGCGAAA20650.060.0471422
CGAAAGC22100.056.431144
GCAGGGT24050.055.465979
GCGTCAG400.006041640454.398481
AGCAGGT66900.053.3166478
GTTAGCC1101.6752892E-952.7357563
AGCAGGG33300.050.7265748
GTGTAGA4650.049.8976026
AAAGCAG138650.048.8438266
GCGAAAG26500.047.611433
GCAGGTA65350.046.149999
AGCAAAA121750.045.800862
Produced by FastQC (version 0.11.5)