FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_99_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_99_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583661
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG24300.072.510635
ACGAGCG1200.072.496899
AACACGA15350.071.3296666
ACACGAG16000.068.418947
TAGTAAC26500.067.048712
GTAACAA27350.064.159314
CACGAGC19050.057.8452878
AGCACGC7400.057.828961
GAAACAC30850.057.115344
TAACAAG32300.054.5513345
ACGAGCA11600.053.7476969
GTAGAAA40850.052.9114071
GCACGCA8250.052.7433242
GCCGAAC1101.6771082E-952.7250148
GTAGTAA35350.052.5261651
AACACGT12900.052.2751056
ACAACGC7750.051.44948
AGTAACA34250.051.238643
ACGAGCC5450.049.2180759
CGGAGCG1351.9099389E-1048.3404165
Produced by FastQC (version 0.11.5)