FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_92_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_92_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677813
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGAGTGCTTTCTGAAAGGTTGATCCCGGGCAAGATAAACGAGGGT23700.1412553127195939No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA22980.1369640120800113No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA22580.13457995616913207No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA18040.1075209215806529No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG17120.1020375929856307No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA17060.10167998459899882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC14100.084.848484
ACACGAG9950.076.5056847
AAACACG15850.075.02295
ACAACGC6000.072.498248
CACGAGC10850.070.159598
AACACGA10950.069.5271456
AACACGT6350.068.5104456
CAACGCA9200.066.1940469
ACACGTG6750.065.516937
TAGTAAC19650.063.841292
ACGAGCA6000.060.41529
GTAACAA20600.060.1877564
TAACAAG23350.053.4097865
AACAACG8500.052.0281527
ACGAGCC4850.050.823519
AGTAACA25850.048.8068663
ACGAGCT2550.048.332169
TAGAAAC31800.046.7460372
ACGTGCC2950.046.6937839
ACGTGCA4200.046.6060149
Produced by FastQC (version 0.11.5)