FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_78_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_78_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059817
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA42880.208173832918167No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA38210.18550191594690207No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT28220.13700246186918547No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC28010.13598295382550976No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT21680.10525206850899863No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA21060.10224209238005125No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG20860.10127113233845532No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT20790.10093129632389673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA11050.091.220112
TAGTAAC49400.088.076972
AGCACGC11750.086.421761
GTAACAA50300.086.206434
GTAGTAA51500.086.194021
CACGCAC12950.080.074423
AGTAACA58900.073.992333
TAACAAG60450.071.8516855
GCGGTGC6050.068.3364641
AAACACG26100.066.677185
ACAACGC8900.065.166158
GAAACAC27350.064.160014
CGCACTT17300.063.290325
AACAACG9950.058.2893187
ACGCACT20550.054.6923184
CGGTGCA3350.054.1169432
GAAGCGG5450.053.23491
CGGTGCG1101.6734703E-952.739422
CGAGCTA1650.052.735585
ACGTGCA9800.052.5376132
Produced by FastQC (version 0.11.5)