FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_740-5_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_740-5_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694678
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20560.29596446123239833No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA16510.23766406882037433No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT15030.21635923406240015No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT14790.21290439599353944No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA13610.19591810882164112No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13330.1918874644079703No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12050.1734616613740467No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10640.15316448771949018No Hit
GTATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAA10530.15158102027126236No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG10450.15042940758164214No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC10420.14999755282303456No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT9870.1420802155818955No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC9800.14107255447847777No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA9580.1379056195820222No Hit
AAAGCAGGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGAGAAG9240.1330112656511362No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC8970.12912457282366793No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA8960.12898062123746543No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC8660.12466207365138957No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8660.12466207365138957No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT8600.1237983641341744No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT8490.1222148966859466No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA8220.11832820385847831No Hit
AGCGAAAGCAGGGAGAGGGGGGTTTAGATAATGTCCTGACCCTGGGCCAG8200.11804030068607327No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG8190.11789634909987073No Hit
GTCCTGACCCTGGGCCAGTGTCTTCAGTGAGCTGCTCCTTGCTTCTGTTC8100.11660078482404798No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG8070.11616893006544039No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC8060.11602497847923786No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG7980.11487336578961764No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT7840.11285804358278223No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA7830.11271409199657971No Hit
AGCGAAAGCAGGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGA7810.11242618882417466No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT7740.11141852772075696No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA7730.11127457613455442No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC7660.11026691503113673No Hit
GGCAGAGAGGCAGGCAGAGAGAGGGAGGCAGAGAGAGGGAGAAGCAGGCT7560.10882739916911145No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCA7380.10623627061746593No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC7340.10566046427265582No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG7270.10465280316923813No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA7180.10335723889341537No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG6980.1004782071693648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9850.0120.740981
GAGCGAA1400.0119.137071
CGAAAGC11650.0101.455953
GCGAAAG12050.099.291662
TCTACTA1150.069.360265
GAAAGCA17100.069.1205754
CGAAGCA451.1925444E-464.455992
GTAGAAA9800.059.1985441
ACAAGGC2450.059.1814968
ACTTAGT753.7529935E-758.0145721
GAGTCCG400.0060509954.3730059
TAGAAAC10650.053.7889862
GCAGGGT4450.050.503768
GTTCCGA450.00963108448.3419956
TAGTAGA1351.9099389E-1048.3419952
TACTAAT1950.048.3315627
GCCCACC604.944927E-448.33156145
CAAGGTA3650.045.683259
AGTAGAA11350.044.724891
AGAAACA13200.043.3979263
Produced by FastQC (version 0.11.5)