FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_562-5_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_562-5_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607059
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG45730.17540838162849404No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA32590.12500676049141962No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA29340.11254060610059073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5850.0112.821871
AGCGAAA12550.076.86271
CGAAAGC13900.070.427683
GCGAAAG13950.070.1752552
GGTTATA2800.062.1671451
TTACGTG2250.061.23442
TACGTGT2300.059.9032253
GTTACGT2350.058.6399351
GGTCGTT1550.056.1272777
ATTCGTG400.00604721254.385822
CGCATAC400.00605272154.3733145
ACTCCGA5850.052.0716321
GCAGGTA11550.050.214889
TCCGAGG6600.049.441653
ATTACGA450.00963328748.342954
TACGCTT901.3225872E-648.342955
CGAGGTG7300.045.6940235
CCGCTCT5400.044.314374
AACGGGT5650.043.6373
CAAGGTA6200.043.2747344
Produced by FastQC (version 0.11.5)