FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_561-5_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_561-5_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573635
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGCGGCAAGGATTGTTATGCCATAGATTAGGGGCCGATGT17180.29949358041263174No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA13180.22976282827930652No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAG12680.2210464842626409No Hit
CTATGATTCTGATCGAGACCCTAGGTATCCATCATAGGAATCGTGGTTTA12310.21459638969030828No Hit
GTCATAGAGTAGTGGTAACACAAAGCAGTTAATTTGTGATTCGTTGTTGT10280.17920803298264576No Hit
AGCGAAAGCAGGGTGCTGCAAAAGTCATATCTATGATTCTGATCGAGACC9660.16839976640198034No Hit
GGTATAGGGAGGGAGGCGATGCCATCATTGGCATTCCGATAGCATCAGCC9400.1638672675133142No Hit
TTTTTTATCAGTGACTTTCAAACACAAATGCAATTAACACCACTCAAACA9180.16003207614598133No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT8330.14521429131764974No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT7830.13649794730098408No Hit
AAAGCAGGGTGCTGCAAAAGTCATATCTATGATTCTGATCGAGACCCTAG7610.1326627559336512No Hit
GATTAGGGGCCGATGTTTCTGTCATAGAGTAGTGGTAACACAAAGCAGTT7600.13248842905331787No Hit
GTGTTGGGAAAGTGGGAGGAGAATGAGTGCACCACTGCTGCTGCTGCTGC7430.12952487208765157No Hit
TTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT7380.128653237685985No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA7060.12307477751531898No Hit
AAAGCAGGCAGGCTGGGCCACTGCGAGGCCCTCTCTTGTGGTGTTTATGC7010.1222031431136524No Hit
GTTCTGAGTTTGCCATTGAAAAATGCGTGGTACCTTTGTCTTCTTAGTCA6820.11889093238731947No Hit
TTCCATAGGCAGTCAAAAATGTCACAAACAACTCAAAGCCACAAATCCGG6780.11819362486598622No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT6450.11244083781498687No Hit
TCCTTAAACTTTAATAACGGCGTTAAGGGCAATAAGATGAGGGTTATGGG6360.11087189589198707No Hit
AGCGAAAGCAGGCCAGGAGCAGCAGGAGGTTCCGGAACACTGGAGGATGA6160.10738535828532081No Hit
GGCTGGGCCACTGCGAGGCCCTCTCTTGTGGTGTTTATGCAAAAGCCGGT6120.10668805076398755No Hit
GAGTAGTGGTAACACAAAGCAGTTAATTTGTGATTCGTTGTTGTGCTTGT6120.10668805076398755No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA5930.1033758400376546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA22100.0122.409661
GAGCGAA3500.0113.970851
GCGAAAG25800.0104.83652
CGAAAGC26900.0100.549513
GAAAGCA29300.093.550814
TATAGGG2500.078.31543
TAGGGAG2750.071.195815
GCAGGCC4850.067.263678
ATAGGGA2950.066.368984
ATTAGTA350.00356049162.1659161
AGCAGGT4950.060.0466047
AAGCAGG51450.060.040966
GAGTACG851.3002136E-859.7280351
AGTACGA851.3020326E-859.7176172
AAAGCAG54850.057.7734345
AGCAGGC14450.057.6952137
GCAGGCG6700.055.1829878
GGTACTC400.006051129754.3714688
CTAATAA400.006051129754.3714689
AAGGTAG4800.052.8749735
Produced by FastQC (version 0.11.5)