FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_561-3_Gr9_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_561-3_Gr9_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915692
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG38110.1989359458618609No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA31130.16250002610022904No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA27330.1426638520179653No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA26720.13947962407318087No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC25610.13368537322283539No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG24610.12846532741171338No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA23860.12455029305337183No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC23050.12032205594636299No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC22280.11630262067179901No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20040.10460971805488564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5650.0120.688911
GTTACGT3250.078.121871
TACGTGT3300.076.91413
TTACGTG3500.072.52282
AGCGAAA10450.068.029571
GCGATTA2250.064.44115145
ACTCCGA5200.062.7764971
CGAAAGC11450.062.702033
GCGAAAG11600.061.894452
AGTCCGC2300.059.888245145
GCAGGTA8500.058.8499379
AGCTACT3850.058.373648
TCCGAGG5750.056.7543
GTTATGT7800.055.8013341
GTCGTCT2000.054.372223145
GGTTATA2300.050.4638181
GCGACTA1750.049.726024
GGTGGGC8500.048.6151668
CCCGCTC6450.047.221683
GTAACTC2000.047.1373563
Produced by FastQC (version 0.11.5)