FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_561-3_Gr5_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_561-3_Gr5_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922723
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG87550.223186801617142No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA86570.22068853701880045No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA55840.14235009711366314No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT52490.1338101109866794No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT50880.12970581914654694No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA50040.12756444949082563No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT49680.12664671963837365No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA48280.12307777021217149No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC47210.12035007315071698No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC46460.11843813595810869No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC44000.11216698196635348No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT42730.10892943498687008No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA42500.10834310758113688No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG42430.10816466010982677No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT42390.108062690126221No Hit
GGATACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG42380.10803719763031955No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC41490.10576836549509104No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG41010.10454472569182173No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC40840.10411135326149719No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG40480.10319362340904518No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT39460.10059338882709792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCT100.007092564144.99373145
GAGCGAA8900.0133.659581
CGCGAAA203.8675743E-4108.74529145
GGACCGC900.0104.7724461
AGGCTCC850.093.81947145
AGCGAAA22400.087.106491
GCGTCAG353.4303644E-582.896881
TGTACGA353.434706E-582.875742
CGTCAGA353.434706E-582.875742
GTACGAC353.4351404E-582.873633
GCGAAAG24450.079.4861452
CCGCATA1200.078.553284
CGAAAGC24950.077.891243
GCAGGTA22700.076.32939
GACCGCA1300.072.516272
CGCATAA1300.072.510735
CGCACGA406.667042E-572.496864145
GCAGGGT36800.071.905859
ACCGCAT1350.069.8287053
GACAGTA3150.069.044637
Produced by FastQC (version 0.11.5)