FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_55_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_55_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919860
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28990.3151566542734764No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC24200.26308351270845565No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA22400.2435153175483226No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA17550.19078990281129737No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG16030.17426564912051834No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT13310.1446959319896506No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT13280.1443697954036484No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT13130.1427391124736373No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC12980.1411084295436262No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA12870.1399125953949514No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC12530.13621638075359294No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT11460.12458417585284717No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT11410.12404061487617682No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA11240.12219250755549757No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC11030.11990955145348205No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG10900.11849629291413911No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG10750.11686560998412801No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA10550.11469136607744655No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10100.1097993172874133No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA9490.10316787337203487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACG100.0070917793144.9931145
ACGTGCG203.8666895E-4108.744839
GAAACAC4950.071.7837754
GTAACAA5150.070.404134
AACACGT1950.066.938086
ACACGTG2050.063.655517
ACGTGCC1450.059.9971479
AAACACG5950.059.719275
TAGTAGA1950.059.506993
TAGAAAC6850.057.1754262
TAACAAG6350.057.0994155
CTCGCCC400.006051871554.372414145
AACACGA4250.052.894216
ACACGAG4250.052.8798377
GGAGTGT553.2153048E-452.7390946
CGGTGCG553.219623E-452.7247667
CACGAGC4300.052.2649578
GAGCAGA3200.052.138071
CGGAGCG858.926945E-751.1740387
AGAAGCG2850.050.8969042
Produced by FastQC (version 0.11.5)