FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_528-3_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_528-3_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156821
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9700.081.494161
GCGAAAG17300.065.808962
AGCGAAA17550.064.4642941
CGAAAGC19750.058.011143
GAGCAAA52900.048.2568241
CAAAAGC63550.039.02134
GCAAAAG65350.037.6162533
CGGGTTG4500.033.837485
AGCAAAA75150.032.904622
TAGTCGG3250.031.2295258
AAAGCAG104600.030.9861096
GTAACGG4750.030.5357191
CGAGGTG6800.028.790295
TCCGAGG7250.028.0053793
GTGCGAT2853.237801E-1027.9813429
AACGGGT5200.027.8899733
GTATACG1050.00769881727.6275541
CCGACTA5300.027.3618434
AAGCAGG119550.026.9249027
CCGAGGT7850.026.786724
Produced by FastQC (version 0.11.5)