FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_286-5_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_286-5_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341626
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGAC10280.30091386486976984No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT7910.23153975399998827No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC7540.22070919660681565No Hit
AGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCG6860.2008043884247686No Hit
GTTTAATACTGTGAACAGCAGCATAGAGTTTAATACTGTGAACAGCAGCA5880.17211804722123022No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA5820.17036174061693196No Hit
ACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGA5520.16158020759544064No Hit
ATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTC5430.15894574768899322No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC5070.14840790806320361No Hit
GGCTTATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTG5010.14665160145890535No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4990.14606616592413926No Hit
GCCCTGGACACTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTT4910.1437244237850749No Hit
AAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTT4840.1416753994133936No Hit
GTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCGGCATCCTCGA4820.14108996387862752No Hit
GGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATC4730.13845550397218012No Hit
TCACTATGCTGCTATTCACTCTCACTATGCTGCTGTTCACAGTATTAAAC4630.13552832629834966No Hit
GTTTTAGTGGTGTTCACTATGCTGCTATTCACTCTCACTATGCTGCTGTT4590.13435745522881748No Hit
CTATAAGCCAACGCGGTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCG4540.13289386639190226No Hit
TTAATACTGTGAACAGCAGCATAGAGTTTAATACTGTGAACAGCAGCATA4480.131137559787604No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC4420.12938125318330573No Hit
TCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGA4420.12938125318330573No Hit
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG4300.1258686399747092No Hit
AGCGAAAGCAGGTGTACAGGTGTACAGGTGTGGATGTTTAATACTGTGAA4280.1252832044399431No Hit
AGCGAAAGCAGGGAGCCATGGCTGAGGGCCTCGATGACGACCAGAAGACG4200.12294146230087874No Hit
GGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCG4070.11913613132489916No Hit
ATGCTGCTGTTCACTCACTATGCTGCTGTTCACTCACTATGCTGCTGTTC3950.11562351811630263No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA3930.11503808258153654No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT3930.11503808258153654No Hit
CACTATGCTGCTGTTCACAGTATTAAACTCACACTATGCTGCTGTTCACT3920.11474536481415348No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3830.11211090490770609No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA3720.1088910094664926No Hit
GTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAGAGTTTAAT3710.10859829169910956No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3700.1083055739317265No Hit
GGTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAGAGTTTAA3670.10742742062957737No Hit
GTGTACAGGTGTACAGGTGTGGATGTTTAATACTGTGAACAGCAGCATAG3650.10684198509481128No Hit
TTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGAT3630.10625654956004518No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3620.10596383179266215No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAA3590.10508567849051302No Hit
AGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGA3580.10479296072312998No Hit
AGCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCG3550.10391480742098083No Hit
TCCCTGAGCGCCGTCGCGGCAAGCAGCGGGCTTGGCCGCATGTCGCTCGA3530.10332937188621476No Hit
AGTAGAAACAAGGTGTTTTAGTGGTGTTCACTATGCTGCTATTCACTCTC3480.10186578304929952No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT3450.10098762974715039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC100.007083528145.03632
AGCGTCA100.007083528145.03633
AGCGAAA9500.0124.444111
GCGAAAG11000.0107.458722
TACTGAT800.099.683277
CGAAAGC11900.099.331593
ACTGATC900.088.607358
GAAAGCA15800.074.8130344
CAGGGGG2850.073.768819
GCAGGGG4050.073.391958
GGCAGAC300.001931603872.528781
AAAGCAG18300.071.329345
AGCAGGG7850.068.341067
GCAGGGC651.3957106E-766.9202358
GAGCGAA2300.066.221921
TAGTAGA554.0498617E-665.925592
AAGCAGG19000.065.266346
AGCAGGT4150.062.88897
CTGGTCA350.00356509362.140229
GCCCACC606.7977453E-660.414104145
Produced by FastQC (version 0.11.5)