FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_199-7_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_199-7_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972872
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA83750.28171411349025455No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA77110.25937880944756453No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG53960.1815079828529449No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA53000.17827878226845958No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC47830.16088819162076268No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG43630.14676043906363948No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA38160.1283607232332909No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC38130.12825981071502573No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA37760.12701522298975534No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT34080.11463662074922835No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA33590.11298838295089732No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC32210.10834640711069969No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA31460.10582359415407054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8050.0114.437391
TACGTGT5750.079.4540253
TTACGTG5950.078.0033952
GTTACGT6300.071.3857041
AGCGAAA13550.066.364052
CGAAAGC13700.065.102634
GCGAAAG15700.063.741553
CAGCAAC22300.063.068997
AGCTACT11100.062.0468378
AACGGGT10850.060.152823
GCTACTA11950.059.4534659
AGTGCAG36100.058.2551083
ACGGGTT11800.055.3062824
CAGTGCA38050.055.079972
GTAACGG11800.054.7101751
ACGTGTT8800.051.0884864
GAGCAAA76950.050.337631
CCCGCTC15000.049.79543
ACTCCGA9950.048.844071
AGTCGGC4900.048.835075
Produced by FastQC (version 0.11.5)