FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_156-7_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_156-7_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413116
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA99410.4119569883917723No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA93480.38738295216641055No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG64690.2680766278952193No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC58180.24109906030211561No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC55130.22845980052347256No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA50930.21105491820534117No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA50120.20769826232970154No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG48680.2017308741063422No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA41610.17243265553748763No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC38060.15772138595906704No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA37810.15668538105917826No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA36190.149972069307899No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT34160.14155970952080216No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA33710.13969490070100235No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG33700.1396534605050068No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC33330.1381201732531714No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT32370.13414191443759854No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA32250.13364463208565192No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30980.12838172719421692No Hit
GACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGATTAAATAAGAAATC30140.12490075073059066No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA29970.12419626739866628No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA29960.12415482720267075No Hit
CTCCCGCTCTGTGTCAAATCAATAAATGACATCTTTAAAAAATAAAGTGA29450.12204137720689763No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG29140.12075673113103556No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACTCTCCATCCAA28290.11723431447141373No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC27790.11516230467163617No Hit
GAGCTGGAGAGAGAGACAAACTGACAGACTGAGCAAAGTGGAAGGAGCAG27600.1143749409477207No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA27540.1141262997717474No Hit
AAATAGAGATCACTTAGAATAGTACCTATGAGAGTAAGCCCTCAAAAAAT25860.10716434684449483No Hit
GCTGTGGAGTAGCAGGGTGGAGGGTGGCAGCACTGGTGGCAGCGGCCTGT25180.10434641351679737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3750.0106.386361
GTTACGT5250.092.569951
ACGTGTT5050.091.896534
TTACGTG5200.090.643892
TACGTGT5450.086.484123
GCTACTA12200.085.570329
AGCTACT13200.079.087728
TATGTGT18050.076.330123
CAGCAAC20300.072.497087
CAGTGCA35650.070.5827262
GTTATGT20650.066.037761
AGTCGGC2000.065.2608955
GATATGT10300.064.085341
AATGGGT14650.063.837027
AGTGCAG39500.063.7018243
AGCGAAA6300.063.306842
TTATGTG22050.061.4980772
TGTGTTG22650.060.506795
GCGAAAG7150.059.8364033
TCTAAGC3450.058.852842
Produced by FastQC (version 0.11.5)