FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l01_n01_102_FLUA_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l01_n01_102_FLUA_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790831
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT76990.27586765375617517No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42460.1521410647939628No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA36060.12920882704828776No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG31290.11211714360346435No Hit
GATATACAGAAGGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA30350.10874897118456832No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT29870.10702905335364271No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG28580.10240677418303008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC17200.092.784081
GCACGCA17500.091.583392
GTAGTAA34900.082.725021
TAGTAAC37000.077.616842
AAACACG27100.074.65765
CACGCAC22450.072.357963
ACAACGC13900.070.41248
AACACGA19350.069.706156
ACGAGCG1900.064.867259
GTAACAA44250.064.2409444
ACACGAG21150.063.0721477
CGCACTT26850.060.7683335
AGTAACA48400.059.4839553
AACAACG16450.058.6159637
CACGAGC23600.056.5244038
AACACGT12850.053.61176
TAACAAG53550.053.0842485
ACGCACT32250.052.1671834
GAAACAC41100.049.2267884
CAACGCA25650.049.1803979
Produced by FastQC (version 0.11.5)