FastQCFastQC Report
Wed 16 Oct 2024
HT3JTDSXC_n02_PilotTMPCRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT3JTDSXC_n02_PilotTMPCRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170516985
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC16172835594.84589174503643No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA5444480.3192925326471143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3332900.1954585345266338No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3314710.19439177862545481No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCTAGC3233920.18965383419135637No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA2946730.17281152373178543No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2041370.11971651973555597No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1998820.1172211671464869No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGT1713360.10048031285563722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)