FastQCFastQC Report
Wed 16 Oct 2024
HT3JTDSXC_n02_PilotResc1CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT3JTDSXC_n02_PilotResc1CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173110013
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC15659506190.45985167825042No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAA36378182.1014486319748586No Hit
CAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGTCTAAA7226470.4174495671720619No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA5247780.30314710911609716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3366040.1944451358801527No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA3195000.18456471376961886No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTCAGC3007480.17373229588978195No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1956220.11300443955255204No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA1933190.11167407167833787No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTTAGC1803050.10415630897098946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)