FastQCFastQC Report
Wed 16 Oct 2024
HT3JTDSXC_n02_PilotD3CRISPR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT3JTDSXC_n02_PilotD3CRISPR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217336030
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC20652867895.02735372501283No Hit
CAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA7205220.3315244140605679No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4576720.2105826631691027No Hit
AAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA4435210.204071547639846No Hit
CAAGTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2702550.12434891720438622No Hit
CAAGTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2652990.12206857740062703No Hit
CAAGTTGATAACGTACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC2595960.11944453020513901No Hit
CAAGTTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTTCAGC2589340.1191399327575828No Hit
CAAGTTTATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGC2269930.10444333597149077No Hit
CAATTGATAACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2251290.10358567790163462No Hit
CAAGTTGATACGGACTAGCCTTATTTAAACTTGCTATGCTGTTTCCAGCA2198470.10115533995904867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)