FastQCFastQC Report
Tue 12 Jul 2022
HT35NAFX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT35NAFX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203156098
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCGTTGG19501478395.99258152713684No Hit
AGATCTCGGT34093021.6781686759902232Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG6282110.30922576589357414No Hit
AAATCGTGGG3217320.1583668928313439No Hit
AAATCGGTGG2238150.11016897952036862No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)