FastQCFastQC Report
Tue 12 Jul 2022
HT35NAFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT35NAFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203156098
Sequences flagged as poor quality0
Sequence length10
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTCGGTA19186139994.44038396524036No Hit
GGGGGGGGGG64463043.1730792545542985No Hit
GCCTCGGTAA6488960.31940759169335886No Hit
GCCTTCGTAA5416620.2666235497395702No Hit
GCTTCGGTAA5219840.2569374018987114No Hit
GCCTTGGTAA2846830.14013017714092935No Hit
CCTTCGGTAA2679530.13189513021656873No Hit
GCCTTCGGAA2100590.10339783155315378No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)