FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_W17_1415_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_W17_1415_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365362
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC212720.8993126633470901No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG133970.5663826509430692No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT113540.4800110934393974No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT86540.3658636606151617No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC78490.3318308149027506No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT69610.2942889925516686No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT67780.28655233321580376No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA63700.26930338781125257No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG63270.2674854842514592No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG58110.24567064153393856No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA57880.24469827451358397No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT57230.24195028076040792No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT56090.23713072248560688No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA55910.23636973960011196No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA55370.23408679094362722No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT55100.2329453166153849No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT54580.23074692161284405No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA51820.21907851736858883No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG51650.21835981131006588No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT51550.21793704304034645No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC51510.2177679357324587No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA50600.21392074447801226No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT50300.21265243966885408No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT49980.211299581205752No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT49930.21108819707089232No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT45790.1935855907045095No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT45380.19185224079866No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG42300.17883097809130272No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC41690.17625209164601444No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT41330.17473012587502462No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA39220.16580971538394546No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT36460.15414131113969026No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT36140.1527884526765882No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA35980.15211202344503716No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT35660.15075916498193512No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT34860.14737701882417997No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC34240.14475585555191975No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA33360.14103549477838911No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA33150.14014768141197836No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG33130.1400631277580345No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA32930.13921759121859573No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG32670.1381183937173253No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG32540.13756879496669008No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC32030.13541267679112118No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC31150.13169231601759054No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG30690.12974758197688133No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC30530.12907115274533032No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC29540.12488574687510834No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA28510.1205312336969986No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA28480.12040440321608278No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG28280.119558866676644No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC27580.11659948878860826No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA26300.11118805493620003No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC25980.10983519647309797No Hit
GAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACTCGTGAA25880.1094124282033786No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG25790.10903193676063115No Hit
GATCAGAAGTCCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAG25610.10827095387513624No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT25270.10683354175809032No Hit
ATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTC25120.10619938935351123No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT24150.10209853713723312No Hit
GCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATT24140.10205626031026117No Hit
CAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTG23950.10125300059779434No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA23730.10032291040441166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)