FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_W17_1411_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_W17_1411_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746905
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG100020.36411889016911764No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT73430.26731903724373435No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG70970.2583635036522923No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT64110.23338994249892153No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC55370.20157231502363568No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA54300.19767702195743939No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT49710.18096730684170004No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA49210.1791470764369354No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT47180.17175694099359096No Hit
GCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCG45970.16735198341406055No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC45160.16440321015834183No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG41360.1505694590821306No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT38680.14081302411259217No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA35250.12832624353590677No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT35190.12810781588733502No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA33420.12166420025446821No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG32740.11918868690398832No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA30080.10950506115064045No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCCTT29660.10797606761063815No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT29510.10742999848920877No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT28950.10539134043587237No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT28700.10448122523349006No Hit
AGCGAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGC28050.10211492570729602No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG27980.10186009345062898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)