FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sp19_2254_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sp19_2254_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801014
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA66910.2388777778333132No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA58400.2084959232620758No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA55120.19678587825694552No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT51710.18461171561441678No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT47830.170759589205909No Hit
CCACATACACTCTTCACATACCCTATACCTCTCTCTTCCCCTCAGAGGTA37760.13480832298589013No Hit
CTTCACATACCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTT34980.12488334581690773No Hit
AAATAACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAG33500.11959954502190992No Hit
CCTATAAATCATCGTATGGTTTATGTCAAAAGTAGTATTCACTAGCATTT29310.10464067655499044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)