FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sp19_2254_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sp19_2254_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661809
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC337371.2674463118878927No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG221380.8316900273460643No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT166670.6261531161702436No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT129740.48741288349389456No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC119940.450595816604422No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT111380.4184372357295358No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA96890.3640005725429586No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT88310.33176685479686935No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA87020.3269205266042755No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT86070.3233515252221328No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA85710.3219990615404787No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT83130.312306405155291No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA82630.3104279833752159No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA79330.29803039962672No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC78620.29536304069901337No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT73020.2743247167621719No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG71850.2699292097967961No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG68560.25756919448390175No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT63760.2395363453951805No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT62840.23608004931984228No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT62510.23484029094499267No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT61410.2307077630288274No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA59680.22420842366976745No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT59640.22405814992736145No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA58610.2201886010604067No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA58510.21981291670439165No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT57410.21568038878822635No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT55910.21004512344800097No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA55100.20700208016427926No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT55000.20662639580826422No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT50700.19047196849961812No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC50060.18806758862112194No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA49660.18656485119706184No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG49430.18570077717822728No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG47350.17788654257311476No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT47260.1775484266527012No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG47240.17747328978149823No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT46990.17653407889146067No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC46190.17352860404334045No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC45660.17153747695646082No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG45230.1699220342255962No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC44510.16721710686228802No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC43490.16338512643093475No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG43330.1627840314613107No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA43140.16207023118488217No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT42840.1609431781168371No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG42700.16041722001841605No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC42460.15951557756398No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40710.15294110133371705No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT40080.15057428989082236No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT39970.15016103709920584No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT39470.1482826153191307No Hit
ATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTC39420.1480947731411232No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC38940.14629148823225108No Hit
GAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACTCGTGAA38040.14291032902811585No Hit
GATCAGAAGTCCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAG37710.14167057065326624No Hit
GGAAAGCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACT37520.1409567703768377No Hit
CAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATTTGCAGACCAAGA36170.13588503157063486No Hit
CAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTG35610.13378119917695072No Hit
GCATTAGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCA35610.13378119917695072No Hit
GCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATT34700.13036247153721398No Hit
TCTTATCTCTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTC33830.12709401763988326No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA33740.1267559017194697No Hit
AAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCTTTC33360.12532830116661264No Hit
ACCAAGAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAG33300.1251028905530036No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC32900.12360015312894353No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT32710.12288635285251497No Hit
TCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGAGACTCGA32630.12258580536770294No Hit
GGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTT32180.12089522576563531No Hit
ACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCT32110.12063224671642482No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA31790.11943005677717672No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA31600.11871625650074819No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT31580.11864111962954517No Hit
GTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGA31460.11819029840232714No Hit
GCTTTCTTTGGCATGTCCGCAAACGATTTGCAGACCAAGAACTGGGTGAT30890.1160488975730415No Hit
GACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAA30440.11435831797097387No Hit
TCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTCCACTTCAA30430.11432074953537238No Hit
GTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCGATGAG30390.11417047579296635No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA29890.11229205401289125No Hit
CCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGC29590.11116500094484616No Hit
ATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGACATCCTCGTCA28980.1088733263731545No Hit
GGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTGGAGCGGATTC28740.10797168391871842No Hit
ATCAAGTGCAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACT28570.10733302051349289No Hit
TTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGAATGGACCAGGCA28010.10522918811980875No Hit
CCCCAATTGCATTTTTGACATCCTCGTCAGTATGTCCTGGAAGGGAAGGT27840.1045905247145832No Hit
TCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCAC27400.10293751354811709No Hit
ATCTTATCTCTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTT27340.10271210293450808No Hit
GCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTG27310.10259939762770355No Hit
CATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGAGACTCGAACT27080.101735323608869No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA26910.10109666020364347No Hit
CATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTGGAGCGGATTCTGG26870.10094638646123745No Hit
GAAAGTGACAGGGCCCCTTTGCATTAGAATGGACCAGGCAGTAATGGGTA26650.1001198808780044No Hit
GGATTGGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCA26650.1001198808780044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)