FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sp19_2235_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sp19_2235_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544267
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG36670.1441279551242067No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG33290.1308431858763251No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT31800.12498688227296899No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC31460.123650544537975No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG26280.10329104610483097No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT26140.10274078939042168No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT25520.10030393822660907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)