FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sp19_2231_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sp19_2231_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047461
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG262001.2796336535836335No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT247701.2097910534071223No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC195190.953327071919807No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT193000.9426308974871804No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG183520.8963296492582765No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC148020.7229441732956086No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC131710.6432845363110702No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA130800.6388400072089285No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA125840.6146148815532995No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT118140.577007327612101No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG107200.5235752964281127No Hit
GCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG106220.5187888804719601No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA104740.511560415558587No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT98730.4822069870927944No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT96380.47072935699385726No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC94780.4629148003307511No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG91240.4456250937136287No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT90820.4435737725895634No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC86890.42437926778580887No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA86270.42135112707885525No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT85720.4186648732259125No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT78360.38271791257562415No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT75690.3696773711440658No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG75220.36738184512427835No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC74020.36152092762694865No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC71440.34891995500769No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA70830.34594065527988077No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG69910.34144728519859474No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG68070.3324605450360227No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG65520.3200060953541972No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT65460.3197130494793307No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT64900.3169779546472436No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT62700.3062329392354726No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG60320.2946087861991022No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG59380.2900177341595273No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA59220.28923627849321676No Hit
AGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC58910.28772220813973987No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT58000.2832776790375983No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT57430.2804937432263667No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG56850.2776609664359907No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG56010.27355832418785997No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC55730.2721907767718164No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC54310.2652553577333097No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG51280.2504565410525524No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC51150.249821608323675No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC49790.2431792351600348No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA49680.24264198438944626No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT49620.24234893851457975No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT49600.24225125655629093No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA49520.24186052872313563No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC48540.2370741127669831No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG48220.23551120143436186No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA48110.2349739506637733No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC47900.23394829010174065No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA47050.2297968068744655No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT46030.2248150270017353No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT45990.22461966308515766No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG45880.22408241231456913No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG45850.22393588937713588No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA45460.22203109119050377No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG45420.22183572727392611No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC45160.22056586181617133No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC44750.2185633816712504No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA44500.21734235719264006No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT43260.2112860757787328No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT42480.20747647940546854No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG42120.20571820415626962No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA41810.20420413380279281No Hit
CTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCA41730.2038134059696375No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG40820.19936887686749588No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC40580.19819669336802997No Hit
AGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC39610.19345911839102184No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG39330.19209157097497828No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG39260.19174968412096738No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT39050.1907240235589347No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC38910.19004024985091292No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA38390.1875005189354034No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG37300.18217685220866234No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG37280.1820791702503735No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT36680.1791487115017087No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA36250.17704854939849893No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT36160.1766089805861992No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC36090.17626709373218832No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG36050.17607172981561064No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG35900.17533911512844447No Hit
GGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGA35870.17519259219101121No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT35580.1737762037958232No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC35510.17343431694181233No Hit
GGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACC35440.17309243008780142No Hit
CAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGC35410.1729459071503682No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG35020.17104110896373606No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA34440.16820833217336006No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG34240.1672315125904718No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG33680.16449641775838467No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT33600.16410568992522934No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC32520.15883086417763267No Hit
GAGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG32490.15868434124019945No Hit
GGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATT32410.15829361340704415No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC31890.15575388249153463No Hit
GGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTA31050.1516512402434039No Hit
TTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCG30540.1491603503070388No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG30120.14710902918297344No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT29970.14637641449580724No Hit
GTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATC29390.14354363770543127No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT28760.14046665601933322No Hit
TTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGC28540.13939215447815612No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC28480.13909910860328964No Hit
CCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTA28300.1382199709786902No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC28120.13734083335409075No Hit
GAGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC27580.1347034204802924No Hit
GGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCAT27430.13397080579312623No Hit
ACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAC27290.13328703208510442No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC27270.1331893501268156No Hit
GATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC27090.13231021250221614No Hit
CTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAG26980.1317729617316276No Hit
AGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG26600.1299170045241399No Hit
ATTCTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGG26560.12972164060756225No Hit
AGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCT26520.1295262766909846No Hit
ACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT26210.12801220633750776No Hit
CTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTC25890.12644929500488655No Hit
GGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACG25880.12640045402574213No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG25860.1263027720674533No Hit
GGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGTAAGGTT25700.1255213164011427No Hit
CACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCA25560.1248375426931209No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGG25470.12439797388082117No Hit
CTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGG25440.12425145094338794No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA25250.12332347233964408No Hit
GGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGT25220.12317694940221083No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG24910.12166287904873403No Hit
ATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCG24850.12136983317386754No Hit
CATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAA24580.12005112673696837No Hit
AAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAA24520.11975808086210188No Hit
GGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGG24250.11843937442520273No Hit
CCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCG24190.11814632855033624No Hit
TCTCTCATCCGCCAAAACATCTTCGGCGTTGTAAGGTTAAGCCTCACGGT23850.11648573525942618No Hit
GTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGT23800.11624153036370413No Hit
TCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGG23690.11570427959311556No Hit
GCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGC22830.11150395538669601No Hit
GAGTAATACAGTGCTATCAAGCTGTTCAAGTGCCATACTGACATACCCTG22560.11018524894979684No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT22260.10872001957546444No Hit
GTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATA22190.10837813272145354No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT22120.10803624586744265No Hit
AGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGT21980.10735247215942086No Hit
ATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAA21860.1067663804096879No Hit
GCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACA21770.10632681159738817No Hit
GATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGC21690.10593608376423286No Hit
CGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCG21400.10451969536904489No Hit
TCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCG21370.10437317243161164No Hit
GCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAG21330.10417780851503398No Hit
GACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT21210.10359171676530103No Hit
GAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCA21130.10320098893214572No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT20920.10217532837011303No Hit
GCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG20910.10212648739096862No Hit
ATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGC20870.10193112347439096No Hit
GCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTG20780.10149155466209125No Hit
GATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATT20700.10110082682893594No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT20690.10105198584979153No Hit
TAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCAC20630.10075893997492504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)