FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sm18_1974_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sm18_1974_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052986
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC190660.9286960554041771No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG128460.625722727773107No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT97080.47287219688785015No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT77960.37973955984112895No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC69600.3390183859022906No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT61700.3005378507208525No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA54710.2664898835160104No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT52030.25343572727724395No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT51400.25036702637036984No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA51260.24968509283550885No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA49170.2395047993507993No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT47150.22966547263352016No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC46120.22464839019847188No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT44150.21505261117221453No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA43650.21261713426199694No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT43140.21013294781357497No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA42200.20555425122236584No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG41740.20331361246496565No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT41020.19980652571425234No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT39870.1942049288207518No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT37880.18451173071808577No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG37500.18266076826632038No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA35910.1749159516918284No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG35360.17223692709058902No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA34720.16911951664551048No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA34310.16712242557913207No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC34130.1662456538914537No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT33590.16361533882841872No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT33520.16327437206098824No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA33460.16298211483176211No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT33000.16074147607436193No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA32040.15606536040674412No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT31960.1556756841011093No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT31950.15562697456290495No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA30610.14909989644352178No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG30560.14885634875250003No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT30150.14685925768612157No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT30030.14627474322766934No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC29810.1452031333871736No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC28830.1404295986431471No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC28660.13960153649367313No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG28140.1370686405070468No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG27650.13468187313503355No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG27570.13429219682939875No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT26890.1309799482315028No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA26670.12990833839100704No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC26620.1296647906999853No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC26120.12722931378976768No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25990.1265960897931111No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC25940.12635254210208935No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC25880.12606028487286322No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT24750.12055610705577145No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG24730.12045868797936274No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA24610.11987417352091051No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG23330.11363935263075345No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT22960.11183709971719243No Hit
ATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTC22800.11105774710592278No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA22740.11076548987669667No Hit
GGAAAGCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACT22570.10993742772722269No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC22270.10847614158109213No Hit
GCATTAGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCA22040.10735582220239204No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA21390.10418970221910914No Hit
GATCAGAAGTCCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAG21300.10375131637526996No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA21270.10360518776065691No Hit
GCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATT21230.10341034960783951No Hit
GAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACTCGTGAA21140.10297196376400033No Hit
CAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATTTGCAGACCAAGA21110.10282583514938728No Hit
TCTTATCTCTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTC21070.10263099699656987No Hit
GCATATGAGAGAATGTGCAACATCCTCAAAGGGAAATTTCAAACAGCAGC21060.10258228745836553No Hit
CAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTG21030.10243615884375247No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC20910.10185164438530024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)