FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_Sm18_1912_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_Sm18_1912_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965332
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA38380.19528507142813528No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA34480.17544109595732427No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT27800.14145192771501203No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA27620.1405360519240515No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT23090.1174865111848787No Hit
AAATAACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAG20350.10354484636692425No Hit
CCACATACACTCTTCACATACCCTATACCTCTCTCTTCCCCTCAGAGGTA19670.100084871156629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)