FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_P2_SynRNA_10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_P2_SynRNA_10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719895
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA165820.6096558874515376No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG140020.5147992845312043No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC115760.4256046648859607No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC109340.4020008125313661No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC104430.38394864507637244No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA75780.278613696484607No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA71450.2626939642890626No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA68610.2522523847427934No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT67290.24739925622128794No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA66650.24504622421086109No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT65390.2404136924403332No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG65340.24022986181451858No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC63470.23335459640905254No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA63370.23298693515742339No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG62860.2311118627741144No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG62340.2292000242656426No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG61980.22787644375977748No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC61470.22600137137646858No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA60130.2210747106046373No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA59750.21967759784844634No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC57840.21265526794232867No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC57330.21078019555901975No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC57310.21070666330869392No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA57080.20986104242994674No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC56940.20934631667766587No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG56460.2075815426698457No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC56210.2066623895407727No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA54230.19938269675851458No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT53220.19566931811705965No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT52530.1931324554808182No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG49700.18272764205971187No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG49230.1809996341770546No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA48830.17952898917053783No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT48400.17794804578853227No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT47360.17412436877158863No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT47350.1740876026464257No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA46350.1704109901301337No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT45700.1680211919945439No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT45690.16798442586938098No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA44460.16346219247434185No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG44280.1628004022214093No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA44040.1619180152174992No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC43940.16155035396587No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT42950.15791050757474093No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC42910.15776344307408927No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG41900.15405006443263436No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC41580.1528735484274209No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA41510.15261618555128048No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC41190.15143966954606702No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA40190.14776305702977505No Hit
CTCTTGATAGTGTCTGTTATTATTCCGTTGTACTTTAACACAGCCACTGC39970.14695420227619083No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG39060.1436084848863651No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT38840.14279963013278085No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA38290.14077749324882027No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC37770.13886565474034843No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT36860.13551993735052273No Hit
TTTTTGAACAGATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCG36470.13408605846916885No Hit
ATGTTAACATCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCC36450.134012526218843No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT36420.13390222784335426No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT36400.1338286955930284No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG35800.1316227280832532No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT35360.13000501857608474No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG35100.12904909932184883No Hit
TTATTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCA34630.12732109143919157No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA34510.12687989793723656No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA34420.12654900281077028No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT34100.12537248680555682No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA34030.1251151239294164No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA33700.12390184179904004No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAA33310.12246796291768616No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC33240.12221060004154573No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC33090.12165910816410191No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA32840.12073995503502893No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA32780.12051935828405141No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT32390.11908547940269754No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT32300.11875458427623126No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA32270.11864428590074248No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA32270.11864428590074248No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA32190.11835015689943913No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA31890.11724717314455153No Hit
CAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA31840.11706334251873694No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT31710.11658538289161897No Hit
CCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTCTTGACTCA31380.11537210076124263No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA31350.11526180238575386No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT31170.1146000121328213No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA31060.11419558475602917No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC30670.11276170587467532No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA30630.11261464137402363No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT30250.11121752861783267No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC30020.11037190773908552No Hit
GCCCCATTGTCTGGGCCAGAAATTCCAATTGTTAGCCAATTGATGCCATC29860.1097836497364788No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG29390.10805564185382156No Hit
ACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG29320.10779827897768113No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA28930.10636440009632725No Hit
CCCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAA28590.10511435184078798No Hit
GTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATG28480.10470992446399585No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT28410.10445256158785543No Hit
CATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGT28300.1040481342110633No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC28000.1029451504561757No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA27980.10287161820584985No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC27970.10283485208068693No Hit
CTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTAT27940.10272455370519819No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT27860.10243042470389482No Hit
CCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCA27610.10151127157482182No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG27410.10077594907156341No Hit
TTTCTATTCTGAAGATCTTGTATGAGGCCTGTCCATCACTTGGTCCATCG27390.1007024168212376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)