FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_P2_MRTPCR_Water_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_P2_MRTPCR_Water_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244315
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATTGTGTCTGGCTTCTTTTGTTTGGCATGATGTCCTCAGAGGTCA10140.4150379632851033No Hit
ATATAGGATAGATGTTCCCTTTCCAAATGGGAGAAATCTGAAAGAGGCGG7500.3069807420747805No Hit
CCTTTAGACCGAGTGATGGTCTGTTGCGTGTCTGTGTCACATTCCGTTTT7430.30411558848208253No Hit
CCCTTACACTTTTCCTGGGACTTCTCAGAACCACCTTCCTAAGCCCCGGC6660.2725988989624051No Hit
TCCTAAGCCCCGGCAGAAGCTCAAGTAGAAAAGAAACCGTCAGTACCAGC5760.23576120991343144No Hit
ATTATATTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTT5620.23003090272803556No Hit
CTATAGGACTGGATAATACCGCTATTTGGCTGCAAATGCTCACATGCTTT5420.2218447496060414No Hit
GTAGTGCAGACAGGGCCAGACGTCAACAGCACTTGAAACCTCCAGGTACT4930.2017886744571557No Hit
CTCTTAACCACTGAAAATGTACAGTTTAAGTCATGGGGATACATCTGCAT4670.19114667539856334No Hit
GTACTGTATCATCATAAAAACTTAAAAGTAATTTTTAAAGCTGTGACCCG4570.18705359883756625No Hit
TTCTTAGCCCTTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA4570.18705359883756625No Hit
TCTCAAGATAGTCTTCACATAACCTGAAACTGGAGCTTCACAGGACCCAA4500.18418844524486833No Hit
TCTCAGAACCACCTTCCTAAGCCCCGGCAGAAGCTCAAGTAGAAAAGAAA4340.177639522747273No Hit
GTCCCAGGACTGTCACCTCCAGAAACTGGAACTCTGTACCCATCGAGCAG4290.17559298446677443No Hit
GGTTAAGAGGGTCCATTTTATGTTATGGGTACTGTATCATCATAAAAACT4220.1727278308740765No Hit
ATCTGAGACTTACTAAAAGACACGTGAATGTGTTTTTTGAATAGTAGTGC4140.16945336962527885No Hit
TTGTATGACCGGTCCCAGGACTGTCACCTCCAGAAACTGGAACTCTGTAC4020.16454167775208237No Hit
CAATAGGACAAGTGGTACGGAATCCTACTCAGAAGAGGTACAGAGAACAG4020.16454167775208237No Hit
CCCTATAGGTGAGGACCAGAGAGGTCTGGTGACTTTTCCCGGGCCACACA4000.16372306243988294No Hit
GTACTGGACTTCTGACTTCTTTTTACTTCTAAGCTCTAGGAGTTAAAAAG3980.16290444712768354No Hit
GTGTAAGGGAGGTCATGAGCCGGAAGAGAAGAGGGTGGTTTCTGGCCTTG3710.15185314041299142No Hit
GGATAATACCGCTATTTGGCTGCAAATGCTCACATGCTTTCTTCAAGAAA3690.15103452510079202No Hit
GTTTAAGTCATGGGGATACATCTGCATCGTTGTATGACCGGTCCCAGGAC3660.1498066021324929No Hit
GTCTAAAGGAACGCTACAACCCGGATGACCTCTGAGGACATCATGCCAAA3620.14816937150809406No Hit
CTCTAGGAGTTAAAAAGAATTTTGATACACGAGGGAATGACATTTTTTGT3360.13752737244950167No Hit
TCACAGGACCCAACAAACTGGAAGTCGGAACTACACTTTGGGGAGATGAT3350.13711806479340197No Hit
GTATGGGTGTGAACCGCGAACTTCATCAGCCCCTTCAGAAGAAGCCAGGA3280.134252911200704No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA3270.13384360354460428No Hit
GTGAAGAAGGTTCTAGAAATGTTGGCCGAGGCTCTTCAAGGCTTCTCCTG3200.13097844995190636No Hit
ATACAGTACCCATAACATAAAATGGACCCTCTTAACCACTGAAAATGTAC3170.12975052698360723No Hit
ACTTTAGATACCTTTCTGTGGATGTAGCATTTGAGGAGAGACATGTAGCT3060.12524814276651045No Hit
TCTGAATACTACGCTAAGGCCTTTCTTGGCTTTCTTTCGGAAAATCTGGA3040.12442952745431103No Hit
GCTAAATACCATGTAAATCAGGTATGGGTGTGACTAGAGGTACAATTCAT3010.12320160448601192No Hit
AGGTCACACTGCTGGCTTTAAACCCAGGTTTCTCTCTGGTACTGGACTTC2990.1223829891738125No Hit
CTCCTAGAGCTTAGAAGTAAAAAGAAGTCAGAAGTCCAGTACCAGAGAGA2950.12074575854941366No Hit
GACCAAGGTCACACTGCTGGCTTTAAACCCAGGTTTCTCTCTGGTACTGG2950.12074575854941366No Hit
CTCTGGTACTGGACTTCTGACTTCTTTTTACTTCTAAGCTCTAGGAGTTA2940.12033645089331395No Hit
TTCTAGAACCTTCTTCACTGGCCCCTTTACAAAAAATGTCATTCCCTCGT2930.11992714323721425No Hit
GTGGAGGGACGATCCCAGGTGTTTCTGAGGAGGCAAGGCCAGAAACCACC2880.11788060495671572No Hit
ACCCAGGGCAGTGGAGGGACGATCCCAGGTGTTTCTGAGGAGGCAAGGCC2880.11788060495671572No Hit
TCCCTCCACTGCCCTGGGTTCAGTTAGTTATTTCTAGAAAGATGCTCTGG2840.11624337433231689No Hit
CCTCAGAGGTCATCCGGGTTGTAGCGTTCCTTTAGACCGAGTGATGGTCT2830.11583406667621718No Hit
CTTCAAGGCTTCTCCTGGGATCTTGCTGCTGGTACTGACGGTTTCTTTTC2780.11378752839571864No Hit
TTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTTCACATA2740.11215029777131981No Hit
TCTCTGTACCTCTTCTGAGTAGGATTCCGTACCACTTGTCCTATTGTGTC2710.1109223748030207No Hit
ATACTACTTTAGATACCTTTCTGTGGATGTAGCATTTGAGGAGAGACATG2700.11051306714692098No Hit
GTCTTATTCCATCCACTCCTGGGTGGGAGTCCTAGCCCTGGCTGGTGGCT2690.11010375949082128No Hit
CTTCTAAGCTCTAGGAGTTAAAAAGAATTTTGATACACGAGGGAATGACA2690.11010375949082128No Hit
TCCCAACATCATCTATAAACCCCAAAGTCTCTATCAGCTAAATACCATGT2670.10928514417862187No Hit
TTATATTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTTC2670.10928514417862187No Hit
GCCCTGGGTTCAGTTAGTTATTTCTAGAAAGATGCTCTGGATGACCTCAA2640.10805722121032274No Hit
CCTTTATTCTTTGCCTACGGAGTCAAGAAATGGTCCGTTTACTCCCTGCT2610.10682929824202361No Hit
GCCTTGAAGAGCCTCGGCCAACATTTCTAGAACCTTCTTCACTGGCCCCT2600.1064199905859239No Hit
CCTTTTGACTACGTTTTGTTGTTTTTAATCTATCAGACCAAGGTCACACT2580.10560137527372448No Hit
GGGCGGGGCTGTGCTTACTGGCCTTTTTCTTGCTGTCCAGTTGGGACTAG2550.10437345230542537No Hit
GTGTGACCTTGGTCTGATAGATTAAAAACAACAAAACGTAGTCAAAAGGT2550.10437345230542537No Hit
CTTCTTCACTGGCCCCTTTACAAAAAATGTCATTCCCTCGTGTATCAAAA2540.10396414464932567No Hit
GTTAGGTGGATTGCTCTCACCTTTTGACTACGTTTTGTTGTTTTTAATCT2540.10396414464932567No Hit
CATCTGCATCGTTGTATGACCGGTCCCAGGACTGTCACCTCCAGAAACTG2530.10355483699322596No Hit
GTCTTCACATAACCTGAAACTGGAGCTTCACAGGACCCAACAAACTGGAA2530.10355483699322596No Hit
GTCCAGTACCAGAGAGAAACCTGGGTTTAAAGCCAGCAGTGTGACCTTGG2520.10314552933712626No Hit
ACCTGAAACTGGAGCTTCACAGGACCCAACAAACTGGAAGTCGGAACTAC2480.10150829871272743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)