FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_FCC_SynRNA_2.5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_FCC_SynRNA_2.5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687480
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA221890.6017388568887153No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG198380.5379825788885634No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC161350.43756169524987254No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA140700.38156139151941165No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC131990.3579409244253528No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT125660.3407747296256522No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC122840.3331272305205723No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC119790.3248559992189788No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC118070.3201915671407031No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG114130.3095067634265135No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT110570.29985247377612895No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA106370.2884625814919674No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG98670.2675811123043379No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT96970.2629709178083678No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC92050.2496284725612071No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA89310.24219792378534932No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT86470.2344961870979639No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT84950.23037413084274358No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG83460.2263334309609815No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG81840.22194018679423344No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG81700.22156052371809476No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG80950.21952661438163734No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA80670.21876728822935987No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT80200.21749270504517992No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA76260.20680790133099028No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA75880.20577738726718517No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC72510.1966383546487032No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA72010.1952824150910649No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA71600.1941705446538015No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG71090.19278748630501047No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA70250.19050950784817816No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT69630.18882814279670668No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT68360.18538405632030547No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT66650.18074674303318256No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA66370.1799874168809051No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC64950.1761365485372124No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT64830.17581112304337923No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC64580.17513315326456008No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC62720.17008905811014569No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA62540.1696009198693959No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA62470.16941108833132654No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA61910.16789243602677167No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC61400.16650937767798063No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA59900.1624415590050658No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGGCTT59280.1607601939535943No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA58600.15891611615520626No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC56110.15216353715816763No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG55990.15183811166433445No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC55950.15172963649972337No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC54250.14711944200375324No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT53890.14614316552225368No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA53790.14587197761072604No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG53560.1452482454142124No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT53320.14459739442654604No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA53040.14383806827426862No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT52970.14364823673619923No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA52640.142753316628158No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC50990.13827871608795167No Hit
ACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG50720.13754650872682697No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG50420.13673294499224403No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG49610.13453632290887002No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT49000.1328820766485513No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA48840.13244817599010705No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG47580.12903120830485862No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT47110.12775662512067862No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG47100.12772950632952584No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT46780.12686170501263735No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT46380.12577695336652672No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCATTG44610.12097692733248723No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA43450.1178311475587664No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC43320.11747860327378046No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGTAG43060.11677351470380855No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA42610.11555316910193411No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA42410.11501079327887881No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG41650.11294976515126862No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT41640.11292264636011584No Hit
CAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA40910.11094297460596397No Hit
TTTTTGAACAGATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCG40690.11034636120060312No Hit
GGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACAGGAGC40330.10937008471910356No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC39110.10606159219846616No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA39080.10598023582500786No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA38970.10568192912232745No Hit
TTACTGTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATC38400.10413615802661981No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC38350.10400056407085599No Hit
GGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGAT37870.10269886209552323No Hit
CCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCA37120.10066495275906581No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA37120.10066495275906581No Hit
CTCTTGATAGTGTCTGTTATTATTCCGTTGTACTTTAACACAGCCACTGC37100.10061071517676029No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT36970.10025817089177433No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT36880.10001410177139942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)