FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_FCC_Seq_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_FCC_Seq_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAAAATGGGCATAATAATAATGCACAACTCCGAGCGTGATGCCACC13.125No Hit
TTTGAGGCCTTCGTTGGAAGCGGGATTTCTCATTTACTGCTAGACAGAAG13.125No Hit
AGGTTGGCCAGGACTAGTCGCTGGCTGGTAAGGGTTCCAGCATTCAAATG13.125No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA13.125No Hit
GCATGATTGAGCATTTTAAGATTTCTCACAAAGGAAGGTTTTTAATTTTA13.125No Hit
CAGGGGGGGTCAGATGCCCTGCAGTGTCTGAAGTGGGGTATGGCCATGGT13.125No Hit
ATCTGGGGGCCGTAGTGCTTGTCCATGCCCGCCAGGATCAGATCGACCTC13.125No Hit
CCTCTGAGGATATTATGAATATTTGCTTTTAGAACAGCTTCTATTTTTAA13.125No Hit
GCTTCTCAGTTAAAGATTTCTGGAATCCATTGCTTCAACGTTCTCATTTG13.125No Hit
TCACACAGCTAGGGGTGGGGATGTATAATCTGACGGGGAAGCGAAGACTA13.125No Hit
GAAGCTGGTGGCTGGATGAGGTACCCAGAGGCCCCGCAATCCACCCAGAA13.125No Hit
AGCAAAAGCAGGCACAGAGGGGCTGGGGAAGTATGCAGACATCGAATTTG13.125No Hit
GTCTCCGAAAATCCCACTACATATGTATCCTATCTGATATTCCAGATTCT13.125No Hit
GTGCTAATGGGGATAATATACCCTCTCTTGCAGATGTGTGGAGGGCCATG13.125No Hit
TTTCTGCCCTCATGTCTGAAGTTCTTCCCTCCGTATTTCCAGTGAATGCT13.125No Hit
AAGCAACACAAAGCAAAAAACATGATATGTCAAAGGAAATCCATAATATC13.125No Hit
GAATTGCTTGAGCCTAGGACGCAGAGGTTGCAGTGAGCAGAGATTGCACC13.125No Hit
CTCATGGAATGGCTAAAGACAAGACAAATCCATTCACCTCTGACTAAGGG13.125No Hit
CATCCACACCATTCTGCTGTTCCTGCCGATACTCTTCACTCATAGACACA13.125No Hit
GTTTCCTGCTAAGGTTCGTAATTCATTGCAGTTTAAGGCTTCATTAGAAG13.125No Hit
ATTAAAGCATTACAAGGATTTATGATTTCTATAAACTGATCACTCATTGA13.125No Hit
GAGCGAAAGCAGGAAGCAGCAGCAGCATAGGAAGAAGAAGAGGAAGAAGA13.125No Hit
GGTCAACTGTTTTCCAGTTCAGATCATGATTCTTCTGTCTACAAGGCAAG13.125No Hit
GCTACTGCCAAAAAAAAAATCCATGATGAATGAAAATGCCCCTTTTTTTT13.125No Hit
GTCTCAACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATT13.125No Hit
GCTGGTACAGTTCTGGTTTGCCACCGGGGGTGCTGGCTTCTGCATCAGCC13.125No Hit
GTGCTCCTAGAATCCAGGCAACACCCAAGTCGATCAGCATTATTATTATA13.125No Hit
CAGTTGTTTCTATATCAAAGGGTCTGGTCTTAACCACTAAGTTGTGCAGA13.125No Hit
GGACCAATATCTTCCAGAGCCTGTCAGCTTTAGCATCCCTCCCATTTCCC13.125No Hit
GTTCATTTCCAAATGAGCCTAGGGATCTCTTGCAAATACATTTTGGTAGT13.125No Hit
GACCCTAAATTCATGACTTGAGCCAAAGGCCAACACATAACACACCAAGC13.125No Hit
GTGATACATACAAAAACTGTTGCTTCTGGTCCTGCATTAAAACTTGAATA13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)