FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n02_New_F17_1800_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n02_New_F17_1800_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401775
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA43430.18082459847404525No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA36540.15213748165419327No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA30200.1257403378751132No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT30000.12490762040574159No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT27330.11379084218963058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)