FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_W17_1415_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_W17_1415_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969974
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA61170.20596139898867802No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA52860.17798135606574333No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA40210.13538839060543964No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT38200.12862065459158903No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT37450.12609537995955522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)