FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_W17_1414_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_W17_1414_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191785
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG110820.5056152861708607No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG85720.3910967544718118No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT69330.3163175220197237No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT68130.3108425324564225No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC64930.29624256028761947No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT55690.25408514065020066No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA54580.24902077530414707No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG54050.24660265491368905No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT53150.2424964127412132No Hit
GCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCG52140.23788829652543475No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA52080.2376145470472697No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC50400.2299495616586481No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT45970.20973772518746137No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT42630.19449900423627317No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA41440.18906963958599954No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA39070.17825653519847978No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT35630.1625615651170165No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT33860.1544859555111473No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA33670.15361908216362463No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG33520.15293470846821197No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCCTT31280.14271472795004983No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC30850.14075285668986695No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT30000.13687473908252862No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG29410.13418286921390557No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA28930.13199287338858512No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT28590.1304416263456498No Hit
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC27400.12501226169537616No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTATC27030.12332413991335829No Hit
AGCGAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGC26920.1228222658700557No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT26760.12209226726161554No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC26720.12190976760950549No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTATCAGACCGCTTCTGCGTTCTG26320.12008477108840512No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG25970.11848789913244229No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTATCAGACCG25190.11492915591629653No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA24690.11264791026492106No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG24200.11041228952657309No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT22810.10407042661574926No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG22730.10370542731152918No Hit
CTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCC22220.10137855674712619No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG22200.10128730692107119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)