FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_Sp19_2254_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_Sp19_2254_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801014
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA75010.2677958767789093No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA64880.23163040241855273No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA59490.21238737114487824No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT54720.1953578239880272No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT45010.1606918066100348No Hit
CCACATACACTCTTCACATACCCTATACCTCTCTCTTCCCCTCAGAGGTA36980.13202361716149938No Hit
AAATAACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAG36380.1298815357581219No Hit
CCTATAAATCATCGTATGGTTTATGTCAAAAGTAGTATTCACTAGCATTT32640.11652922834373552No Hit
CTTCACATACCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTT31570.11270918317437899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)