FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_Sp19_2239_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_Sp19_2239_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765985
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC137340.4965319768545383No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT58770.21247403727785943No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG53530.19352961060887894No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT48300.17462133742590794No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC47130.1703913795627959No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC45490.1644622078572371No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC44830.16207607778060978No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA37200.1344909679553577No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT34660.12530798250894348No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA34520.1248018337048104No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC34130.12339184775043972No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT33910.1225964710582306No Hit
GCATATGAGAGAATGTGCAACATCCTCAAAGGGAAATTTCAAACAGCAGC33600.12147571299193596No Hit
ACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAACAGGTA33190.11999342006554627No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA32130.11616115054853876No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT31870.11522115991229165No Hit
GATCAGAAGTCCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAG31800.11496808551022511No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA30170.10907506729067584No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC30080.1087496859165903No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA29660.1072312395041911No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT29020.1049174163995828No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT28480.10296512815506952No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG28400.1026759002669935No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA28290.10227821192088894No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA27900.10086822596651825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)