FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_Sp19_2232_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_Sp19_2232_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799455
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG42670.15242252509863527No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG41640.14874323752301785No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA37400.13359743235736957No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG33880.12102355637079361No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33490.11963042806546274No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT32630.11655840154601521No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC32220.11509383076348789No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA29080.10387736184364457No Hit
GTGTATCATCTGTTCATTCTGGAGAAAAATAAATGTGATCTCTTTTTGTT28010.10005518931363426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)