FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_Sm18_1914_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_Sm18_1914_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209527
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA22010.18197196094010304No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA19900.16452712506624492No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT15150.12525557511324675No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA14800.12236188195881531No Hit
ATTTGAGAGAGAGCACAAGCAGGTGGAGGGGCAGAGGGAGAGGGACAAGC13890.11483827975729355No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT13260.10962963207931696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)