FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_Sm18_1912_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_Sm18_1912_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965332
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA42680.21716432643441413No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA38830.19757476090553655No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT29290.14903334398462956No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA29050.1478121762633489No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT22710.11555299562618426No Hit
AAATAACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAG21090.10731011350753969No Hit
CCTATAAATCATCGTATGGTTTATGTCAAAAGTAGTATTCACTAGCATTT19820.10084810098242943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)