FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_P2_SynRNA_10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_P2_SynRNA_10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719895
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA147790.5433665637827931No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG140390.5161596311622324No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC121060.4450907112223082No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC117580.4322960996656121No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC94120.346042770033402No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA79460.29214363054456144No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA75260.2767018579761351No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA73630.2707089795745792No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT70320.25853939214565264No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA69280.254715715128709No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT68880.2532450701221922No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG67320.2475095545967767No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG64610.23754593467762544No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA64480.23706797505050747No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA64220.23611205579627154No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG64180.23596499129561987No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG63800.2345678785394289No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC60870.22379540386669336No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA60650.22298654911310917No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC60460.22228799273501368No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC60020.22067028322784518No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC59580.2190525737206767No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC59490.21872167859421043No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC57620.21184641318874442No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG55550.20423582528002No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA55210.2029857770244807No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC55040.20236075289671107No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT54700.2011107046411718No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA54060.19875767263074495No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT52910.19452956823700915No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG52570.1932795199814699No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA51540.18949260908968912No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT49170.18077903742607712No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG48980.18008048104798163No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT46890.17239636088893137No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT46530.17107278038306625No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA46390.17055805463078538No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA46240.17000656275334158No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC45910.16879328062296522No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC45350.16673437761384172No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT44860.16493283748085863No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT44730.1644548778537407No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT43870.16129299108972955No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG43580.16022677346000488No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA43560.16015324120967905No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG43320.15927085420576897No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA41970.15430742730877478No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT41130.1512190727950895No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA41050.15092494379378615No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC40710.1496748955382469No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA39860.1465497748993987No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA39380.14478500089157853No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC39200.14412321063864597No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG39000.14338788813538758No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC38660.1421378398798483No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT38630.14202754150435953No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT38200.140446598122354No Hit
ATGTTAACATCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCC38080.14000540462039893No Hit
TTTTTGAACAGATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCG37360.13735824360866872No Hit
CTCTTGATAGTGTCTGTTATTATTCCGTTGTACTTTAACACAGCCACTGC37270.13702734848220244No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA36920.13574053410150025No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT36910.13570376797633732No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT35750.1314388974574386No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA35740.13140213133227568No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC35630.13099770395548357No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT35380.13007855082641057No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT35220.12949029282380387No Hit
CAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA35000.12868143807021962No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG34970.12857113969473088No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC34830.12805641394245No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA34310.12614457543397817No Hit
TTATTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCA34270.12599751093332645No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA34080.125298954555231No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC33970.12489452717843888No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT33930.12474746267778719No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG33590.12349741442224793No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT33550.12335034992159624No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT33510.12320328542094457No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA33220.12213706779121988No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA32690.12018846315758513No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA31850.11710010864389986No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA31800.11691627801808525No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA31350.11526180238575386No Hit
ACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG30950.11379115737923708No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT30670.11276170587467532No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA30470.11202638337141692No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC30210.111070464117181No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT30140.11081310124104056No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA30130.11077633511587764No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAA30080.11059250449006303No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA29830.10967335136099005No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC29790.10952628686033836No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT29730.10930569010936084No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA29670.10908509335838333No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA29650.10901156110805749No Hit
TTACTGTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATC29540.10860713373126536No Hit
CCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTCTTGACTCA29520.10853360148093952No Hit
CTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTAT29500.10846006923061369No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA29360.1079453434783328No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG29330.10783504510284403No Hit
CAATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGT28890.10621733559567556No Hit
CATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGT28710.105555545342743No Hit
GAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACC28320.10412166646138912No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT28240.10382753746008577No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC27820.10228336020324313No Hit
CCCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAA27560.10132744094900722No Hit
GCCCCATTGTCTGGGCCAGAAATTCCAATTGTTAGCCAATTGATGCCATC27550.10129067482384431No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT27380.10066565069607467No Hit
CCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCA27200.1000038604431421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)