FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_P2_MRTPCR_Water_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_P2_MRTPCR_Water_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244315
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATTGTGTCTGGCTTCTTTTGTTTGGCATGATGTCCTCAGAGGTCA8870.36305589096044044No Hit
ATATAGGATAGATGTTCCCTTTCCAAATGGGAGAAATCTGAAAGAGGCGG8190.33522297034566034No Hit
CCTTTAGACCGAGTGATGGTCTGTTGCGTGTCTGTGTCACATTCCGTTTT7360.30125043488938463No Hit
TCCTAAGCCCCGGCAGAAGCTCAAGTAGAAAAGAAACCGTCAGTACCAGC6390.26154759224771296No Hit
CCCTTACACTTTTCCTGGGACTTCTCAGAACCACCTTCCTAAGCCCCGGC6240.25540797740621735No Hit
ATTATATTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTT6040.24722182428422323No Hit
GTAGTGCAGACAGGGCCAGACGTCAACAGCACTTGAAACCTCCAGGTACT5700.23330536397683319No Hit
CTATAGGACTGGATAATACCGCTATTTGGCTGCAAATGCTCACATGCTTT5120.20956551992305014No Hit
ATCTGAGACTTACTAAAAGACACGTGAATGTGTTTTTTGAATAGTAGTGC5070.20751898164255164No Hit
GTCCCAGGACTGTCACCTCCAGAAACTGGAACTCTGTACCCATCGAGCAG5040.20629105867425251No Hit
CAATAGGACAAGTGGTACGGAATCCTACTCAGAAGAGGTACAGAGAACAG4900.20056075148885658No Hit
CTCTTAACCACTGAAAATGTACAGTTTAAGTCATGGGGATACATCTGCAT4740.1940118289912613No Hit
GTACTGTATCATCATAAAAACTTAAAAGTAATTTTTAAAGCTGTGACCCG4660.19073736774246364No Hit
TCTCAAGATAGTCTTCACATAACCTGAAACTGGAGCTTCACAGGACCCAA4570.18705359883756625No Hit
TTCTTAGCCCTTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA4390.17968606102777152No Hit
GTTTAAGTCATGGGGATACATCTGCATCGTTGTATGACCGGTCCCAGGAC4310.1764115997789739No Hit
GGTTAAGAGGGTCCATTTTATGTTATGGGTACTGTATCATCATAAAAACT4250.1739557538423756No Hit
TTGTATGACCGGTCCCAGGACTGTCACCTCCAGAAACTGGAACTCTGTAC4200.17190921556187708No Hit
GTCTAAAGGAACGCTACAACCCGGATGACCTCTGAGGACATCATGCCAAA3990.16331375478378324No Hit
TCTCAGAACCACCTTCCTAAGCCCCGGCAGAAGCTCAAGTAGAAAAGAAA3940.16126721650328468No Hit
GGATAATACCGCTATTTGGCTGCAAATGCTCACATGCTTTCTTCAAGAAA3810.1559462169739885No Hit
CCCTATAGGTGAGGACCAGAGAGGTCTGGTGACTTTTCCCGGGCCACACA3670.1502159097885926No Hit
TCACAGGACCCAACAAACTGGAAGTCGGAACTACACTTTGGGGAGATGAT3630.14857867916419376No Hit
GTACTGGACTTCTGACTTCTTTTTACTTCTAAGCTCTAGGAGTTAAAAAG3490.14284837197879788No Hit
ATACAGTACCCATAACATAAAATGGACCCTCTTAACCACTGAAAATGTAC3480.14243906432269818No Hit
GTGTAAGGGAGGTCATGAGCCGGAAGAGAAGAGGGTGGTTTCTGGCCTTG3450.14121114135439902No Hit
TTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTTCACATA3290.1346622188568037No Hit
CTCTAGGAGTTAAAAAGAATTTTGATACACGAGGGAATGACATTTTTTGT3240.13261568057630518No Hit
CCTCAGAGGTCATCCGGGTTGTAGCGTTCCTTTAGACCGAGTGATGGTCT3210.13138775760800606No Hit
ACCCAGGGCAGTGGAGGGACGATCCCAGGTGTTTCTGAGGAGGCAAGGCC3170.12975052698360723No Hit
GTGAAGAAGGTTCTAGAAATGTTGGCCGAGGCTCTTCAAGGCTTCTCCTG3130.1281132963592084No Hit
CTCCTAGAGCTTAGAAGTAAAAAGAAGTCAGAAGTCCAGTACCAGAGAGA3120.12770398870310867No Hit
TCTGAATACTACGCTAAGGCCTTTCTTGGCTTTCTTTCGGAAAATCTGGA3100.12688537339090927No Hit
GACCAAGGTCACACTGCTGGCTTTAAACCCAGGTTTCTCTCTGGTACTGG3100.12688537339090927No Hit
ACCTGAAACTGGAGCTTCACAGGACCCAACAAACTGGAAGTCGGAACTAC3080.12606675807870985No Hit
GACTTAAACTGTACATTTTCAGTGGTTAAGAGGGTCCATTTTATGTTATG3040.12442952745431103No Hit
CTCTGGTACTGGACTTCTGACTTCTTTTTACTTCTAAGCTCTAGGAGTTA3040.12442952745431103No Hit
CTTCAAGGCTTCTCCTGGGATCTTGCTGCTGGTACTGACGGTTTCTTTTC3020.12361091214211162No Hit
TCCCTCCACTGCCCTGGGTTCAGTTAGTTATTTCTAGAAAGATGCTCTGG2950.12074575854941366No Hit
ATACTACTTTAGATACCTTTCTGTGGATGTAGCATTTGAGGAGAGACATG2900.11869922026891512No Hit
AGGTCACACTGCTGGCTTTAAACCCAGGTTTCTCTCTGGTACTGGACTTC2890.11828991261281542No Hit
GCTAAATACCATGTAAATCAGGTATGGGTGTGACTAGAGGTACAATTCAT2880.11788060495671572No Hit
TTCTAGAACCTTCTTCACTGGCCCCTTTACAAAAAATGTCATTCCCTCGT2860.1170619896445163No Hit
GTATGGGTGTGAACCGCGAACTTCATCAGCCCCTTCAGAAGAAGCCAGGA2840.11624337433231689No Hit
TCCCAACATCATCTATAAACCCCAAAGTCTCTATCAGCTAAATACCATGT2820.11542475902011748No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA2810.11501545136401778No Hit
CTTCTTCACTGGCCCCTTTACAAAAAATGTCATTCCCTCGTGTATCAAAA2790.11419683605181837No Hit
CCTTTTGACTACGTTTTGTTGTTTTTAATCTATCAGACCAAGGTCACACT2740.11215029777131981No Hit
CTTCTAAGCTCTAGGAGTTAAAAAGAATTTTGATACACGAGGGAATGACA2720.1113316824591204No Hit
TTATATTCCTGTCCTTAGTAATTTCAGAATGGCATCTCAAGATAGTCTTC2710.1109223748030207No Hit
GCCCTGGGTTCAGTTAGTTATTTCTAGAAAGATGCTCTGGATGACCTCAA2690.11010375949082128No Hit
GTGGAGGGACGATCCCAGGTGTTTCTGAGGAGGCAAGGCCAGAAACCACC2670.10928514417862187No Hit
GTCTTATTCCATCCACTCCTGGGTGGGAGTCCTAGCCCTGGCTGGTGGCT2670.10928514417862187No Hit
ACTTTAGATACCTTTCTGTGGATGTAGCATTTGAGGAGAGACATGTAGCT2670.10928514417862187No Hit
GTCTTCACATAACCTGAAACTGGAGCTTCACAGGACCCAACAAACTGGAA2640.10805722121032274No Hit
GTCCAGTACCAGAGAGAAACCTGGGTTTAAAGCCAGCAGTGTGACCTTGG2560.10478275996152507No Hit
GTGTGACCTTGGTCTGATAGATTAAAAACAACAAAACGTAGTCAAAAGGT2540.10396414464932567No Hit
GGATAGATGTTCCCTTTCCAAATGGGAGAAATCTGAAAGAGGCGGCAGGT2530.10355483699322596No Hit
GAATATAACTGGGTCCCCACGAGGCTTCTGAAAGAAGTGTTGTCTGAGTG2490.10191760636882713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)