FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_FCC_Seq_Neg_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_FCC_Seq_Neg_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAGGTCCAATCGACCGAAGGAGAGCCGGGAAATGGGTGAGAGCGC13.125No Hit
GTAGAGAGTAGTGCAAACCAGAATGCAGACCTAGCATGACAAAACCATAA13.125No Hit
GAATATAGAATAAAGAATCTCCTTGTTAAACTTGCATTGTAGACAGAAGA13.125No Hit
CCTTGAACTAATGCGTTTAGTTCTCCCTATCCAAACACAATTGCCGTATT13.125No Hit
CGCCAGAATTACAAGGAGAGAAGTGCCTCTGTTGGAGGAATGTTTGGTTG13.125No Hit
GTCTATGTTAGGTGCTGCTCTAAAATTAAAAACCTTCCTTTGTGAGAAAT13.125No Hit
ATGAGGACAATTGGGACTCACCCTAGATCCACTGCACGTCTAAAAGATGA13.125No Hit
CACAAGAGTCAGCTGGTGTGGATGGCATGCCATTCTGCTGCATTTGAAGA13.125No Hit
CCCAATGCCTGAGCCACCCAGGCGGCCCCCAGGCTCAATTCTTAAAACTT13.125No Hit
GCTCTCATTCATTCTATCATTGTTATGTGTTAGAAGATTTCTTAATATCA13.125No Hit
CTTACCTCCTGGACCCGGCTCCCTTCCTGAGTTGCCACGACTGTTGTGGC13.125No Hit
GGAGCATAATAATGCTGATCGCCTAGGGTCTTGCCAGGATTCAAGGCGCA13.125No Hit
GCCAGATCAGCGACTCGATTCCAGGACACAGAGCACATTACATCACCCTA13.125No Hit
GCAGAAACTGGTGCCGGAGCTTCCCCTGAAGACGCTCAAGCACAGCGACA13.125No Hit
CATTTTAATGTGTACATGAATTTATATTCTTCGTTAAAATGAAGATTCTG13.125No Hit
TTATAAACTAGGAGATACTGCCCAATAATGAAAATGATTATGCAAACACC13.125No Hit
GAGCAAAAGCAGGGGGAGAAGAATGGTGGGAGTCCTGGTAATTTTTTTCT13.125No Hit
GGCGTATAAATGGTGAGGATTTTGTAAGTTCCCTCAGCTTCCAGCGTAAC13.125No Hit
CAATTAGAATTTCTGGCCCAGACAATGGGGCAGTGGCTGTGTTAAAGTAC13.125No Hit
CAGAGAGAGAGGAGGAAGCAGGCTCCCCACTGAGCAGAGAGCCCGATGTG13.125No Hit
AGCCTGCACCATTTGCCTAGCCTGACTTGCGACTTCCATGGCTTCTGCTG13.125No Hit
CCCCGGCTGTCAGACACAGCCTTCCTCTGAGGACCCAGGGACGATGGGGT13.125No Hit
GGCTAGCAGATCATGGAGGTAGAGCATTTCTAGGCAGTCTCCTGCTTTAA13.125No Hit
CGCCTGATTTTTATTCTTTTTGTTTTTTAAATATTTTATTTATTTGACAC13.125No Hit
GTTTCAACTCTGCTCTCTCTAAAGAAAGGTTCAACTCTGTGAGTTGAGTA13.125No Hit
TCTGACCCCCAGGCCAGCATAGTAGGTGTGAGGACAGGTCCAGGCAGAAA13.125No Hit
CCACTGTACTTCCTGGCCATGTGGTATTTGGCAAACTGTCATATGTGTCA13.125No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGA13.125No Hit
CTTCACTCAGGGGCCTCTAGGGCTATCCCAGTACAAAGGCCACCTCTTCC13.125No Hit
ATACATTATACCGCGATCGTTTTGGTCTTCTAATGAAGCCTTAAACTGCA13.125No Hit
CATCAGATTGAAAAAGAATTCTCAGAAGTAGAAGGGAGAATTCAGGACCT13.125No Hit
GTTCATATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTC13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)