FastQCFastQC Report
Thu 12 May 2022
HT335BGXL_n01_New_F17_1800_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT335BGXL_n01_New_F17_1800_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401775
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA46090.19189974081668765No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA38780.16146391731115528No Hit
CCCTATACCTCTCTCTTCCCCTCAGAGGTAATGACATTTTTTTTTATTCA32540.13548313226676104No Hit
ATATATAATTAGAAAATCAAGCAAATGCAGGAGGCCCAGGTGACTCAGTT27810.11578936411612245No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT27100.11283321709985322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)