FastQCFastQC Report
Thu 20 Feb 2025
HT2WFDRX5_n02_BS_SMART2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT2WFDRX5_n02_BS_SMART2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57694771
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA9610491.6657471437056228No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT7984891.383988507381371No Hit
GGCTTAGACAGCTAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA1192440.20668077528204418No Hit
CTTCTGATACGTCCAGCAGCCCTCTCGAGCCACCTTCATCCGCTTACAGA778250.13489090718463898No Hit
GTCTAAGCCTTCCCACTTCGTTTCCCACTTAATATAGACTTTGGGACCTT672570.11657382260863813No Hit
GTATTGCTACTGATGCCGCAGCTTCGGTGCTATATTTGAGCCCCGTTACA631150.10939466247296484No Hit
GACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCCGGC599060.1038326332901122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)