FastQCFastQC Report
Thu 20 Feb 2025
HT2WFDRX5_n02_BS_SMART2_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT2WFDRX5_n02_BS_SMART2_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53570505
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACATCTGCTGCATTAATAGTAGCCGCGACATTATCTTCGTTTC697240.13015371051663596No Hit
GTGTATACCACTCGATTATGTCGTTCAGATGTTCCTTTTGTTTCTCGTTG680190.12697098897985No Hit
GGTATGCCTGCTCGCCCTCGTCGCTGTCGTCTATGTGTCTGCAAAGCCAC577880.1078727930602857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)